Calculation of unfolding energy of DNA hairpin with MFOLD/UNAFOLD

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Bin
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Joined: 10/24/2017

I'm wondering how to use MFOLD for the calculation of unfolding energy of DNA hairpin with MFOLD or UNAFOLD. The unfolding energy of DNA hairpin refers to the free energy required to break the base pairs in the hairpin stem sequentially. Several pieces of literature (PNAS, 2006, 103, 6190-6195;Nat. Commun. 2014, 5, 5167) reported the use of MFOLD/UNAFOLD for the calculation.
Also, is there any free trial version of UNAFOLD available?
Thanks.