dG: melt.py vs. UNAfold.py

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rhizotron
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Joined: 11/19/2012

Hi there,

What do the dG values in melt.py represent? I noticed that these values are not the same as those reported by the .det file created by UNAfold.py, which appears to be for hairpins. I presume melt.py is accounting for additional sources of free energy -- if so, what are these?

My goal is to screen many thousands of probes based on quality, and dG is one of my parameters. Which calculation of dG would be better to use?

Thanks for your help,
Erin